The Incorporation of C14-Adenine and C14-Phenylalanine by Developing Root-Tip Cells
نویسندگان
چکیده
منابع مشابه
The incorporation of formate-C14, glycine-2-C14, adenine-4, 6-C14, and phosphate-P32 into nucleic acids.
nine labeled either with C'4 or N'5. Considerable controversy has developed as to which one of these tracer compounds gives an accurate measurement of the “relativeturnover-rate― of the nucleic acids. In order to determine the “absolute turnover rate― of the nucleic acids, it is necessary to know the specific activity of the immediate precursor of the nucleic acids. Since even the ident...
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Allium cernuum, Vicia faba, and Tradescantia paludosa were treated by root immersion in maleic hydrazide (1 mM/liter) labeled with C(14) (C(14)-MH) for 1 hour to determine the location within the cell to which MH moves during various periods of time after treatment. Root tips were fixed 24 hours, 48 hours, 72 hours, and 3 weeks after treatment. Autoradiographs of root tips squashed 24 to 72 hou...
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Previous studies on the physiological aspects of violacein biosynthesis (DeMoss and Evans, 1959) suggested that only L-tryptophan would serve as the primary source of carbon for pigment formation by Chromobacterium violaceum. In view of the pigment structure suggested by Ballantine et al. (1958), 5-[3-(5-hydroxyindolyl)]-3-(3-isatinyl)-2-pyrrolone, it seemed advisable to determine the actual so...
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A previous study has shown that under suitable conditions typhus rickettsiae can incorporate small but significant quantities of labeled methionine. For maximal uptake, adenosine 5’-triphosphate, glutamine, all of the naturally occurring amino acids, the mononucleotides, diphosphopyridine nucleotide, glutathione, and a soluble protein fraction from normal yolk sac tissues were required (1). In ...
متن کاملThe liver nucleic acid incorporation of adenine-8-C14 during azo dye carcinogenesis.
Brown and associates (2, 9) conclusively demon strated that isotopically labeled adenine was in corporated into the purines of the nucleic acids of normal tissues. The results of these and other in vestigators indicated that the uptake of the labeled compound into the cytoplasmic ribonucleic acid (RNA) was considerably greater than that into the desoxyribonucleic acid (DNA). In re generating li...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1957
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.43.12.1038